Study on flavonoid and bioactivity features of the pericarp of Citri Reticulatae ‘chachi' during storage

The mature pericarp of Citri Reticulatae 'Chachi' (PCRC) is one the six traditional Chinese medicinal materials that should be used after long storage, and it was regarded longer medicine stored, better. However, aging mechanism not clear. To further investigate effect on main active flavonoids PCRC, ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) metabolomics analysis were to analyze PCRC stored for different periods. In results, 219 detected. 5,7,3?,4?,5?-pentamethoxy dihydroflavone 2?-hydroxy-3,4,5,3?4?,6?-hexamethoxychalcone found from first time. According clustering metabolites, times 0 year, 1 2 year clustered into group, 3 4 29 other group. Quantitative showed former group contained a greater amount than latter while 15 polymethoxyflavonoids. newly harvested compared 5 groups (stored 1, 2, 3, 4, years). Eight flavonoids, tectochrysin, apigenin, 2?-hydroxyisoflavone, luteolin, 6-hydroxyluteolin, gallocatechin, quercetin -O- acetylhexoside apigenin ?7-O-[?-D-glucuronide (1 ? 2) -O-?-D-glucuronide], as marker components discriminate PCRC. addition, antioxidant potency composite index (APC) indicated three or four years had stronger activity By means molecular docking, reviewed antiviral against SARS-CoV-2 in freshly significantly higher results this study supplied scientific data quality control, evaluation, rational utilization basic information metabolic regulation